Convertible Debentures Essay Example | Topics and Well Written Essays - 250 words

Convertible Debentures - Essay Example Some small companies are not able to meet the financial parameters for raising equity; thus, convertible debentures are issued instead of selling shares of stocks (Market Outbox par.3). Another motive of corporations for issuing convertible debentures is that they are able to raise equity without giving up control of ownership until such time that the debentures are converted into shares of stock. Furthermore, with debentures, the assets of the company will not be tied up unlike other forms of debt which require assets as collateral for the loan (Business Finance ,par.2). It is believed that issuing convertible debentures is a good alternative for companies who want to raise capital in short term intervals. It is win-win solution for both the issuer and the investor. It is attractive to investors because of the guaranteed interest payments and an option to convert to equity if the price of the stocks goes up. The company on the other hand is able to enjoy lower interest payments and can offer their shares at a premium based on the present value in the

Entrepreneurship Essay Example | Topics and Well Written Essays - 500 words - 4

Entrepreneurship - Essay Example Architecture for Humanity is one such institute. It was started in1999 by two individuals, Cameron Sinclair and Kate Stohr, out of their entrepreneurial spirit (Sinclair, 11). The initial concept was to search for architectural remedies to some humanitarian calamities and avail design services to populations in need. They planned to create openings and avenues through which architects and designers all over the globe would respond to humanitarian catastrophes. To achieve this, they planned to work through various forums and organize various activities such as collaborations with other firms and groups, workshops and competitions. However, regardless of various undertakings since 1999, they had not succeeded in building a single structure till after the September 11, 2001 terrorist attacks on World Trade Centre (Sinclair, 11). They had humble beginnings and for four years were working from a small office cubicle in New York that was only 4 sq. ft. Motivated by their vision of responding and alleviating the suffering of people in humanitarian crises, they undertook various activities and ventures most at times they were strained resource-wise. First, they launched a competition to design provisional housing units for refugees in Kosovo in which they planned to build few units for refugees in Kosovo. The plans went well and raised a substantial amount of cash as well as creating awareness and building new affiliations (Sinclair, 13). Though they never constructed the houses for the refugees due to restrictions by the Kosovo government, the money went to charity through another organization- War Child. Other projects they have undertaken venture include outreach program – a Programme to fight HIV/AIDS in sub-Saharan Africa through mobile clinics (Sinclair, 13). Other activities include response to both natural and artificial debacles such as earthquakes, floods, catastrophes caused by war and other human conflicts. Design Corps is

Clinical Applications of Cone Beam Computed Tomography

Clinical Applications of Cone Beam Computed Tomography Abstract The present article evaluates various clinical applications of Cone Beam Computed Tomography (CBCT). Among scientific articles, a research was conducted by PubMed on dental application of CBCT, containing many articles in general, among which most of them were clinically about dentistry and its related analyses. Different functionalities of CBCT, including oral and maxillofacial surgery, root treatment, implantology, orthodontics, temporomandibular joint dysfunction, periodontics, and forensic dentistry have been indicated in a study. This review article illustrates that different CBCT indicators have been used concerning the need for certain discipline of dentistry and the kind of conducted procedure. Introduction Two-dimensional imaging techniques in dentistry have been employed since the first intraoral radiography was created in 1896. Since then, dental imaging techniques have evolved by the advent of tomography and panoramic imaging. While tomography makes it possible to divide the desired levels from an X-ray range, panoramic imaging provides a comprehensive observable image of maxillofacial structures(1). Recent developments of digital diagnostic imaging has been dealing with lower radiation doses and faster processing times, without affecting the diagnostic quality of intraoral and panoramic images. Two-dimensional images, however, have their own natural limitations (including enlargement, distortion, and folding images), which cause the structures to appear erroneously(1). Cone Beam Computed Tomography (CBCT) is capable of producing 3D images, which leads to effective diagnosis, treatment, and further advances. By introducing dent alveolar imaging in 1998, CBCT could produce lower-cost and lower absorbed dose 3D data in comparison to conventional CT(2). CBCT imaging technique is based on a cone-beam X-ray, gathered on a two-dimensional recognizer, with the privilege of achieving more radiation. In contrast to the conventional CT, a parallel change from the recognizer system is not required during the spinning, which brings about a more efficient use of tube power(3). Being compared with the resultant slideshow images of the conventional CT, the cone shaped radiation spins around a certain object once (in this case was the patients head and neck) and is capable of producing hundreds of 2D images from a certain anatomical volume(4). Then, using different kinds of algorithms that are made by the Feldkamp in 1994, the images are reconstructed in a 3D observable data set(5). Compared to a common 2D radiography, CBCT has various advantages, including no folded images, measuring ratio of 1:1, no geometric distortion, and 3D demonstration. It is worth mentioning that, by using a relatively low ionic radiation, CBCT provides a 3D representation from hard tissues along with little information from soft tissues(6). Common CT systems have similar advantages (in addition to providing information on soft tissues), however, they create the image call with higher levels of ionic radiation and longer scanning time. In total, larger CT units will cause them to be a weak alternative for the Dental offices(7). Applications in oral and maxillofacialsurgery The resultant 3D CBCT images have been used to investigate the right place and the maxillofacial pathology area, as well as assessing the final impact or the additional tooth and its link with vital structures(8-23). These images have been utilized to look into the bone graft space, before and after the surgery and osteonecrosis of the jaw changes (such as those who were exposed to bisphosphonates), as well as the pathology and/or paranasal sinus defect(24-28). Moreover, CBCT technology was applied to assess patients with obstructive sleep apnea to adopt an appropriate surgery method (if required)(29). Since CBCT units were available extensively, dentists have made use of this technique increasingly to investigate maxillofacial injuries. In addition to preventing form folded images, which appear in panoramic images, CBCT made it possible to precisely measure the surface intervals, as well(30, 31). This distinct advantage caused CBCT to become an established method for the evaluation and management of mid-face lesions and orbital fractures, assessment of fracture, observation of maxillofacial bones engaged in surgery, and routing during operation along the processes that are related to gunshot(32-37). CBCT is widely used in orthognathic (orthodontic surgery) and orthmorphic surgeries, in a way that the details of intraocclusalrelationships and the display of tooth surface are vital for adding a 3D skull model. Using advanced software, CBCT made it possible to slightly observe the soft tissues and enable the dentists to control posttreatment beauty, as well as assessing the outline of lips and bone area of the palate in patients with palatal split(38-43). Applications in root treatment While several studies have shown that high contrast CBCT images could be used to distinguish between apical granuloma and apical cysts with measuring dental trauma, yet CBCT imaging is an applicable tool for the diagnosis of periapical injuries(44-46). Other scholars use CBCT as a useful tool to classify the origin of damages, including root or non-root origin, which indicates another period of the treatment(47). The reliability of theses labels (root or non-root) are doubtful. Consequently, they are the foundation of demand on (more) non-invasive techniques for the diagnosis of damages that are usually detected through non-invasive processes. Several clinical sample reports have concentrated on using high resolution CBCT images to diagnose the vertical fractures of the root(45, 46, 48, 49). CBCT is considered a salient technique for periapical radiographs in diagnosing root vertical fractures, measurement of dentin fracture depth, and detecting the root vertical fracture(50, 51). CBCT imaging has made the early diagnosis of inflammatory root resorption possible, which is slightly detectable by 2D radiography(52, 53). As well as detecting the root and cervical root resorption (internal and external), CBCT is also capable of recognizing the extent and progress of the injury(54-58). CBCT could be used to identify the number and morphogenesis of roots and their related canals (both main and supplementary), and also determine the functional length, type, and angle size(54-56). CBCT performs a more accurate evaluation of root canal resorption than 2D imaging(48). It also applies in identifying the extent of pulp in talon cusp and the position of damaged tools(59, 60). Due to its simplicity and precision, CBCT is utilized in canal preparation with different tool techniques, as well(61, 62). CBCT is a pre-operation tool for figuring out the proximity of tooth to the adjacent vital structures, make the surface anatomy right size and cause extent determination to become possible(63-65). In emergency cases after the injury, in which it is vital to recognize the desired tooth status, CBCT images could help dentistry with a selection of the best treatment methods(66, 67). Applications in dental implants As the need for dental implant, as an alternative to the lost tooth, increased helping the treatment plan and avoiding the damage to vital adjacent surfaces during the operation requires for a technique to get the right cavity and measure the position of implant. Previously, such measurement was generally provided by 2D radiographs (in special cases) that was obtained through conventional CTs. CBCT, however, is an appropriate option for dental implant, which in comparison with 2D images, provides more precision in measurement and lower radiation dose at the same time(68-80). The new software lowers the chance of improper settling of accessories and damaged anatomic structures(81-84). CBCT decreases the implant failure by providing information on bone density and cavity shapes, as well as the height and width of the proposed implanting space for patient(85, 86). CBCT does not calculate the Hounsfield scale accurately; hence, the number of bone density through this technique could not be vertical through a group of CBCT units or patients. However, the effect of CBCT in measuring and evaluating the cavity shapes has brought about the selected improvements. By a prior notice about the complications, which could occur during a proposed treatment, the plan can be designed in a way that resolves them or results in an alternative treatment. CBCT is usually used in post-operation evaluation to assess the bone graft and implant position in the cavity(79). Orthodontics applications Orthodontics, in introducing qualitative software of evaluation such as Dolphin (Dolphin ]maging Management Solutions) and In Vivo Dental (Anatomage), enables the dentists to fully exploit the CBCT images for cephalometric analysis. Moreover, it is an appropriate tool for investigating the amount of facial growth, age, function of respiratory tract, and disrupting the destruction of tooth(87-92). CBCT is a reliable tool to evaluate the amount of damaged tooth proximity to the vital structure, which could interrupt the orthodontic procedure(93, 94). When the mini-implant[1] is required as a temporary holder, CBCT provides the observable guidelines for accurate and safe installation and thus, accidental and fatal injuries could be avoided(95-97). Accordingly, the evaluation of bone density before, during, and after the treatment indicates that whether or not the injury has decreased or remained unchanged(98, 99). CBCT illustrates different aspects of maxillofacial complications in one scan. In addition to 3D structure of skeleton bones, it enables the dentist to access anterior, crowns, and axial images. These images could be turned to allow the dentist to observe patterns and various angles of the image, including those that are not available in 2D radiography(100, 101). CBCT images are capable of auto-correction for enlargements and creating vertical images by measurement ratio of 1:1. Consequently, CBCT is more accurate than panoramic and conventional 2D images(102). Applications in TMJ disorders TMJ (temporomandibular joints) diagnostic images are vital for to accurately detect diseases and joints malfunction. According to Tsiklakis et al., though CT is easily available, it is not prevalent in dentistry due to high required costs and doses. Examining the right linking space and position of condyle in the cavity has been made possible by CBCT, which is a tool for showing probable dislocation in a connecting disk(103). CBCT precision and lack of folded images make the measurement of the roof of the glenoid fossa and observation of soft tissue around TMJ possible, which can provide a practical diagnosis and eliminate the need for MRI(104-106). According to Tsiklakis et al., MRI â€Å"is one of the most useful tests since it provides images from both soft and bone tissues†(103). While MRI is recommended for evaluation of TMJ soft tissues, CBCT has lower radiation dose. However, it is emphasized that CBCT technique, unlike CT and MRI, does not reveal the details of soft tissues. The aforementioned advantages made the CBCT the best imaging tool for incurred injuries, fibrous ankylosis, pain, dysfunction, cortex erosion of Cortical condyle, and cyst(107-109). Applications in periodontics As Vandenberghe et al. believe, 2D radiography is the most prevalent imaging used in the bone morphology, such as a defect in periodontal bones. The limitations of 2D radiography, as a result of probable errors and misconceptions in indentifying reliable reference anatomic points, forced dentists to estimate the amount of lost or existing bone(110). These findings approve the observations achieved by Misch, in which the 2D radiography is for identification of alterations in bone level or the architecture of inefficient bone defect(111). CBCT provides an accurate measurement of intrabony defects, by which doctors are able to assess the amount of rupture, valve defects, and periodontal cyst(112-114). While CBCT and 2D radiography are compatible with revealing interproximal defects, it is only the 3D images, such as CBCT, that are able to illustrate the buccal and lingual defects(115). To obtain the details of morphologic of bone features, CBCT is used with precision as the direct measurement with a periodontal probe(110, 111). Moreover, CBCT could be utilized to express the performance derived from periodontal defects and enable the doctors to assess the results of post-periodontal surgeries(115). Application in forensic dentistry Age estimation is one of the significant aspects of forensic dentistry. In this process, is it vital for doctors to be capable of estimating the age of every person in a legal system (including those who have passed away). This is one of the specific cases in Europe and as Yang et al. declared in 2006, â€Å"every year thousands of under-aged people flee over the all European countries with no formal ID card to find a shelter and protection. On top of this, most of the crimes are committed by people, who seem to be under-aged. In either case, it is necessary to determine the chronological age and fill them in documents, similar to those we have seen in Belgian that are under-aged and want to enjoy ethnic and social benefits.† The text of the present article was published for age estimation in line with the relationship between tooth change and age. The tooth enamel, beyond a natural cover, is extremely safe against such major alterations. However, as the age raise the pulp com plex (dentin, cementum, and pulp) illustrates the physiological and pathological changes(116). Usually, the extraction and section cut is required to identify morphological changes, which are not always observable. Nevertheless, CBCT is a non-aggressive alternative. Discussion Since late 1990s, when this method entered dentistry, CBCT scanners have shown substantial advances in medicine and maxillofacial imaging(117). This review article indicated that recent articles were conducted on CBCT, most of which were designated to clinical applications. Most of these articles are about oral and maxillofacial surgery, root treatment, dental implant, and orthodontics. CBCT has limited functionality in restorative dentistry, which is due to its higher radiation dose than 2D radiography and its incapability in providing additional diagnostic information. Moreover, these researches are mostly in the field of restorative dentistry for exploring various privileges of CBCT. Although this review did not assess any related articles to prosthetic applications of 3D scanners, yet the standard surveillances that were conducted in prosthetic treatment could be contingent to the use of CBCT with other dental specialties. For instance, dental implant prosthetic, maxillofacial prosthetic, and TMD evaluation are applicable, which in turn by unifying the resultant data of patients with treatment plan can increase the success of prosthetic treatment. CBCT images embrace issues with medical complications, especially in cases that several teeth and bone levels should be evaluated. New CBCT systems can be utilized in specific dentistry applications. They have higher resolution power, as well as lower exposure and cost in comparison to the prior existing systems. While CBCT has various advantages over 2D radiography, there are natural limitations to this technique that require more precise consideration in the selection of criteria and indices. For example, CBCT is sensitive to removable dentures (including removable dentures peculiar to CT technology) and stiffener bars around a compact object. Overall, CBCT has low contrast and limited strength in viewing internal soft tissues. Most modern CBCT units have flat panel detectors, which are mostly inclined to the bar of stiffening artifacts and are able to provide more information. However, due to the lack of compatibility between artifacts, CBCT is not capable of precise HU measurements; therefore the bone density measurement is not reliable. We believe it is vital to take the principle of â€Å"As Low As Reasonably Achievable†, (ALARA), into consideration. The belief should not be mistakenly interpreted as a reason to avoid the use of high dose CBCT units, which provide us with credible information. There is no tough protocol concerning when the technology must be used and every dentist, oral radiologist and neuroradiologist, must actively assess his/her operational protocols. Image resolution needs an extensive knowledge of anatomy in the fields, which are commonly the domain of dentistry and neuroradiology. Accurate knowledge and experience is required for the clarification of scanned data that determines why imaging is needed. Also the clarification of implicit findings is illustrated, which are explicit in the scan beyond the common scopes of dentistry, including disorders that can be observed in any adjacent area. The fact that CBCT promotes the specialized knowledge and improves the standards of dental care is something that dentists must define case by case. Such an evaluation calls for continuous training and education for dentists and scholars. The recent upsurge in the popularity of CBCT caused many units with low variation (sometimes important though) to be resulted in uncontrolled and unobserved report of the radiation amount. This unapproved report could be due to the limited technological knowledge of medical imaging apparatus in the new units. In response, the academy of European dentistry and maxillofacial radiography has established basic principles for dental applications of CBCT. Summary Based on what has been proposed in this article, most dental CBCT applications are for oral and maxillofacial surgery specialists, root treatment, dental implant, and orthodontics. CBCT test should not be taken unless it is necessary and do more good than harm. While using this method, the whole image dataset (which is a radiology report from a dental surgeon, neurologist, or a general radiologist familiar with the head and neck anatomy) should be assessed completely to maximize the resultant clinical data and make sure that every significant implicit finding were reported. Further researches should be concentrated on the resultant accurate data regarding doses of CBCT systems in which they comprise of a size detector and a background, limited from the scanned volume and sight. CBCT systems with larger background and less metal artifacts for orthodontic and orthognathic surgeries are not available yet. Further evaluations are required for better determination of CBCT applications in forensic dentistry. [1] Implant with less than 3 millimeter diameter

The Machiavellian Element in Shakespeares Julius Caesar Essay

Machiavelli's Moral and social philosophy, as expressed in the prince, and the way this is related to in the political philosophy, style, and actions of Julius Caesar of Shakespeare's play For the reason that philosophy including all other branches of knowledge, from head to toe, is meant for the welfare and wellbeing of mankind thus the sacred branch of knowledge such as philosophy is all about discovering and investigating the hidden for the further wellbeing of mankind instead of putting the same human beings into the hands of totally inhuman structure based on Machiavelli's moral and social philosophy. I would rather call Machiavelli's moral and social philosophy as mere tactics of treating human being worse than live-stock. If people are still firm on calling Machiavelli’s recommended tactics as philosophy then, better to say, at the end of the day we will end up with egg on our face. Frankly speaking, a rather illogical viewpoint as given by Machiavelli can not be called as philosophy at any cost. Historically, Machiavelli was an Italian political theorist whose book The Prince (1513) describes the achievement and maintenance of power by a determined ruler indifferent to moral considerations. Niccolo Machiavelli (1469-1527), Italian author and statesman, is one of the outstanding figures of the Renaissance, b. Florence. Machiavelli's best-known work, Il principe [the prince] (1532), describes the means by which a prince may gain and maintain his power. His â€Å"ideal† prince (seemingly modeled on Cesare Borgia) is an amoral and calculating tyrant who would be able to establish a unified Italian state. The last chapter of the work pleads for the eventual liberation of Italy from foreign rule. Interpretations of The Prince... ... Machiavelli, Niccolà ³. 1560; facs. 1969. The Arte of Warre. Trans. Peter Whitehorne. Amsterdam and New York: Da Capo. Shakespeare's Machiavelli, August 5, 2003, http://web.uvic.ca/shakespeare/Library/SLT/ideas/machiavelli2.html "About Machiavelli" Section, August 5, 2003, http://www.niccolo-machiavelli.com/about.html Machiavels, August 5, 2003 http://www.shakespeare.com/queries/display.php?id=3355 "The Qualities of the Prince" by Ron King, August 5, 2003, http://www.geometry.net/detail/philosophers/machiavelli_nicolo.html Julius Caesar by William Shakespeare, Review by Edward Tanguay November 15, 1996 http://userpage.fu-berlin.de/~tanguay/book50.htm Niccolo Machiavelli, August 5, 2003, http://www.smuc.ac.uk/English/en251/en251_5.htm Concerning Liberality And Meanness, August 5, 2003, http://www.geocities.com/vitomonti2002/juliuscaesar4.html

Investment Property Essay

The objective of this Standard is to prescribe the accounting treatment for investment property and related disclosure requirements. Investment property is property (land or a building—or part of a building—or both) held (by the owner or by the lessee under a finance lease) to earn rentals or for capital appreciation or both, rather than for: (a) use in the production or supply of goods or services or for administrative purposes; or (b) sale in the ordinary course of business. A property interest that is held by a lessee under an operating lease may be classified and accounted for as investment property provided that: (a) the rest of the definition of investment property is met; (b) the operating lease is accounted for as if it were a finance lease in accordance with IAS 17 Leases; and (c) the lessee uses the fair value model set out in this Standard for the asset recognised. Investment property shall be recognised as an asset when, and only when: (a) it is probable that the future economic benefits that are associated with the investment property will flow to the entity; and (b) the cost of the investment property can be measured reliably. An investment property shall be measured initially at its cost. Transaction costs shall be included in the initial measurement. The initial cost of a property interest held under a lease and classified as an investment property shall be as prescribed for a finance lease by paragraph 20 of IAS 17, ie the asset shall be recognised at the lower of the fair value of the property and the present value of the minimum lease payments. An equivalent amount shall be recognised as a liability in accordance with that same paragraph. The Standard permits entities to choose either: (a) a fair value model, under which an investment property is measured, after initial measurement, at fair value with changes in fair value recognised in profit or loss; or (b) a cost model. The cost model is specified in IAS 16 and requires an investment property to be measured after initial measurement at depreciated cost (less any accumulated impairment losses). An entity that chooses the cost model discloses the fair value of its investment property. Fair value is the price that would be received to sell an asset or paid to transfer a liability in an orderly transaction between market participants at the measurement date. An investment property shall be derecognised (eliminated from the statement of financial position) on disposal or when the investment property is permanently withdrawn from use and no future economic benefits are expected from its disposal. Gains or losses arising from the retirement or disposal of investment property shall be determined as the difference between the net disposal proceeds and the carrying amount of the asset and shall be recognised in profit or loss (unless IAS 17 requires otherwise on a sale and leaseback) in the period of the retirement or disposal.

Membrane Permeability

The Neurophysiology of Nerve Impulses and Effects of Inhibitory Chemicals on their Action Potentials Aferdita Sabani Biol 2401. C5L Dr. Endley March 20, 2013 Introduction Cell structure and function can be defined in many aspects but one the most important characteristic is that it is enclosed within a cell membrane called a plasma membrane. The plasma membrane is by-layer composed of lipids and embedded proteins. This membrane is semi-permeable due to its hydrophobic and hydrophilic regions.At the boundary of every cell the plasma membrane functions as a selective barrier that allows nutrients to be brought in and/or removed from inside the cell. The cells permeability and transport mechanisms allow for this occurrence and it is vital for a functional and healthy cell. Transport through the plasma membrane occurs in two basic ways: passive and active processes. The passive transport process is driven by the concentration or pressure differences between the interior and exterior envi ronment of the cell.According to Kenyan college biology department, â€Å"Simple diffusion is when a small non-polar molecule passes through a lipid bilayer. It is classified as a means of passive transport. In simple diffusion, a hydrophobic molecule can move into the hydrophobic region of the membrane without getting rejected†. Particles diffuse passively through small pores within the plasma membrane and they also move from an environment of high concentration towards an environment with lower concentration. Osmosis is a type of diffusion when it comes to water transport.Both diffusion and osmoses move substances down their concentration gradient. Facilitated diffusion is also passive transport, but does not involve the simple movement through pores and lipid dissolving. In this case a carrier protein in the membrane is introduced to facilitate the transport of substances down their concentration gradient. Active transport is not passive because energy in the form of cellu lar ATP is required to drive the substances across the membrane, therefor the cell must spend some f its energy to get through or move against the concentration gradient. In one type of active transport the substance gets across the membrane by forming a substrate –enzyme complex where the substance is picked up by a carrier protein and are then able to move into cell. This combination is lipid and large so energy is needed to defy opposing forces. According to Pearson/biology, â€Å"Active transport uses energy to move a solute â€Å"uphill† against its gradient, whereas in facilitated diffusion, a solute moves down its concentration gradient and no energy input is required. If an experiment was conducted where the conditions of transfer were manipulated by adding in larger membrane pores, increasing protein carriers, increasing pressure and adding higher levels of ATP for active transport the rates of transfer will increase providing an optimal level of reactions. E xperimental Methods and Materials In conducting this experiment the materials needed were a computer the PhysioEX 8. 0 C D and the Anatomy and Physiology Lab Manual because this was a computer simulated experiment. Activity One: Simple DiffusionTwo beakers were placed next to each other and joined by a membrane holder. Four membranes were used and each possessed a different molecular weight cut off (MWCO) consisting of 20, 50, 100, and 200 MWCO; and were tested using NaCl, Urea, Albumin, and Glucose solutions. First, the 20 MWCO membrane was placed in the membrane holder between the beakers and the first solute studied was NaCl. A 9mM concentrated solution was dispersed into the left beaker and the right beaker was filled with deionized water. This transfer was allowed 60 minutes.At the end of this time lapse the results were recorded (see result section of the report). The 20 MWCO membrane was removed and each beaker was flushed for the next run. A membrane with the 50 MWCO was pla ced between the beakers and the steps performed above were repeated using the 9 mM NaCl solution for 60 min. and then repeated again for the 100 and 200 MWCO, as described by the A & P Lab Manual by Marieb and Mitchell. The next solutions tested were Albumin, Urea, and Glucose. All were placed into the left beaker independently and the tests were run exactly like that for NaCl.Activity Two: Facilitated Diffusion In this experiment the set-up of the two beakers and membrane holder was used again. Only NaCl and Glucose solutes were used and membranes with 500, 700 and 900 glucose carrier proteins The 500 membrane was placed between the beakers and the glucose solution with a concentration of 2. 00mM was delivered to the left beaker. The right beaker was filled with deionized water. The timer was set for 60 minutes. When the time was up the data was recorded and the beakers were flushed to set up for the next run.The same steps were repeated using the 2. 00 mM glucose solution with the 700 and 900 carrier protein membranes, separately for 60 minutes. The last run of this transport mechanism was done by increasing the 2. 00mM to 8. 00mM glucose concentration. This experiment was done the same way as above for each of the 500, 700 and 900 carrier protein membranes for 60 min. respectively. Activity 3: Osmotic Pressure In this experiment pressure readers were added in order measure osmotic pressure change and were placed on top the two beakers.A 20 MWCO membrane was placed between the beakers and a NaCl concentration of 8mM was put into the left beaker. Deionized water was placed into the right beaker. Time was set at 60 minutes. The pressure steps were repeated with the 50, 100 and 200 MWCO membranes Activity 4: Active Transport This experiment resembled the osmosis experiment except that an ATP dispenser was substituted for the pressure meters on top of the beakers. In this experiment it was assumed that the left beaker was the inside of the cell and the right bea ker was the extracellular space.The membrane used had 500 glucose carrier proteins and 500 sodium-potassium pumps. Membrane was placed between the beakers and a NaCl concentration of 9. 00mM was delivered into the left beaker and a KCl concentration of 6mM was dispensed into the right beaker. The ATP was the changing variable in this experiment. 1mM of ATP was dispensed and transfer was observed for 60 min. It was observed when no ATPmM was applied and finally when 3mM ATP was applied. Results Activity 1: Simple Diffusion TABLE 1 Dialysis Results (average diffusion rate in mM/min) Solute| Membrane (MWCO)| 20| 50| 100| 200| NaCl| No diffusion| 0. 0150| 0. 0150| 0. 0150| Urea| No diffusion| No diffusion| 0. 0094| 0. 0094| Albumin| No diffusion| No diffusion| No diffusion| No diffusion | Glucose| No diffusion| No diffusion| No diffusion| 0. 0040| NaCl had no diffusion until the 50 MWCO was introduced and then it had a constant rate through the larger pored membranes. Urea diffused at 1 00 MWCO and up. Albumin had no diffusion through any of the membranes and Glucose diffused only through the 200 MWCO membrane. Activity 2: Facilitated Diffusion TABLE 2Facilitated Diffusion Results (glucose transport rate (mM/min) | Number of glucose carrier proteins| Glucose concentration(m/M)| 500| 700| 900| 2. 00| 0. 0008| 0. 0010| 0. 0012| 8. 00| 0. 0023| 0. 0031| 0. 0038| As the number of glucose carrier proteins increased so did the rate of transfer for both concentrations of glucose. The higher concentration of the 8. 00 m/M had a faster rate than that of the 2. 00 m/M glucose concentration Activity 3: Osmotic Pressure TABLE 3 Membrane (MWCO) | Solute| 20| 50| 100| 200| Na* Cl-| 272| 0| 0| 0| Albumin| 136| 136| 136| 136|Glucose| 136| 136| 136| 0| The osmotic pressure was highest and only occurred with the 20 MWCO membrane. Albumin had a constant pressure of 136 mm Hg with every membrane and Glucose had constant pressure of 136 mm Hg until it was relieved when the 200 MWCO mem brane was introduced. Activity 4: Active Transport Table 4 Run: 1 Solute| ATP| Start Conc. L| Start Conc. R| Pumps| Carriers| Rate| Na*| 1. 00| 9. 00| 0. 00| 500| ——–| 0. 0270| K*| 1. 00| 0. 00| 6. 00| 500| ——| 0. 0180| Glucose| ———| 0. 00| 0. 00| ——-| 500| 0. 0000| Run: 2 Solute| ATP | Start Conc. L| Start Conc.R| Pumps| Carriers| Rate| Na*| 0. 00| 9. 00| 0. 00| 500| ——–| 0. 0000| K*| 0. 00| 0. 00| 6. 00| 500| ——-| 0. 0000| Glucose| ———-| 0. 00| 0. 00| ——–| 500| 0. 0000| Run: 3 Solute| ATP | Start Conc. L| Start Conc. R| Pumps| Carriers| Rate| Na*| 3. 00| 9. 00| 0. 00| 500| —–| 0. 0050| K*| 3. 00| 0. 00| 6. 00| 500| —–| 0. 0033| Glucose| ———| 0. 00| 0. 00| ———| 500| 0. 0000| When 1 ATP was dispensed the Na and K transported at a higher rate than when 3 ATP was dispense d and there was no transport when ATP was absent. Discussion Activity 1: Simple DiffusionUpon observing the results for all of the solutes with the 20 MWCO membrane between the left beaker and the artificial external environment of deionized water in the right beaker no diffusion occurred, because the pores were not large enough for them to pass through. An observation that is important to note is that even the small ions of NaCl did not diffused here, so it is obvious that the other molecules would also not diffuse. At 50 MWCO the pores were just large enough for the dissociated NaCl ions to get through but the threshold stopped there because Urea, Albumin and Glucose molecules in the solute were too large.Observations of the diffusion of the solutes with the 100 MWCO membrane showed that all but albumin and Glucose passed, so urea size was now compatible for the size of this pore. Finally, when the 200 MWCO membrane was introduced everything except Glucose got through because it i s a very large molecule that cannot diffuse simply. It must be facilitated. Activity 2: Facilitated Diffusion In the facilitated diffusion of Glucose the parameters that were introduced were the number of carrier proteins available for transport in the membrane.According to the results, when there was a 2. 00mM concentration of Glucose in the left beaker there was evidence of diffusion based on the measured rate of diffusion in mM/min. As the number of carrier proteins increased by 200 between 500 and 900 the rate between 0 . 0008 to 0. 0012mM/ min also increased by 0. 0002 min into the beaker. When 8. 00mM of Glucose was placed in the left beaker with the same carrier protein membrane criteria of 500, 700, and 800 the rate increased. The rate was actually faster than that of the 2. 00 mM concentration.As the concentration of glucose raised the demand for the protein attachment increased so more carrier proteins got involved, while previously some were just hanging out because there was less glucose to transfer. Activity 3: Osmosis In this experiment the study was based on the transfer of water across a membrane. Osmosis of water tends to balance out concentrations, so it will flow to an area of higher solute concentration. Water flowing to a more concentrated solution will usually increase in volume but in this closed system for the experiment the focus was on the increase of pressure.The solutes were confined to their area by a semi-permeable membrane based on the pores of the membrane and the size of the molecules in the solute. With 8mM of NaCl with a 20 MWCO membrane the pressure reading was 272 mHg because the salt was not able to pass through the membrane, but the water diffused to the salt side so there was pressure causing and unequal balance, but with the membranes of 50, 100 and 200 MWCO there was no pressure because the membrane became permeable to the salt allowing an equilibrium between he beakers, therefore no pressure. In the case of Albumin, t he water diffused building up pressure until there was no more water left to diffuse so pressure remained constant at all MWCOs. The same occurred with Glucose until the membrane was replaced with the 200 MWCO membrane. Glucose was able to diffuse thus resulting in equilibrium in both beakers. Pressure will rise until equilibrium is obtained. Activity 4: Active TransportThe experiment showed that at 1 ATP the reaction took place at very slow rate and not completely. Without ATP the transfer didn’t take place at all. When 3 ATP’s were added transfer took place quickly and almost completely. The more ATP introduced to the cell, the faster and more complete the transport will occur which is very important for the transport of glucose since it is a substrate for the production of more ATP.